Tag Archives: Whole genome mapping

Survey Result: Have You Heard About Whole Genome Mapping?

In our latest poll we asked you about your knowledge and experiences with whole genome mapping.

As you can see in the pie chart there is no clear answer to that question. But most of you have heard about it and like the technology behind. For all of you who haven’t heard about it I can recommend you our recent blog posts about whole genome mapping using the OpGen technology:

For our next survey we would like to know your opinion about comparing different NGS technologies. I look forward to your answers.

What is Optical Mapping?

Whole Genome Mapping (WGM) using the OpGen Argus technology delivers high resolution, ordered whole genome restriction maps from single DNA molecules. To receive such a restriction map it is crucial to isolate long DNA fragments (200 kb in size and longer) and to capture the DNA on a solid phase. Afterwards the DNA is digested revealing restriction cleavage sites as gaps when using a fluorescence microscope to visualize the DNA. This optical map will then be converted into digital data, the so called “single molecule restriction maps” (see video below). The software MapSolver enables the following analysis options (see details in the analysis video):

  • Perform Genome Comparisons
  • Identify Motifs, Annotate Features, and view in silico sequence data
  • Perform Sequence Placement
  • Create Similarity Clusters

 Video about step 3: How to scan and assemble single molecule restriction maps (SMRM)

Recently we gained access to this innovative technology and are able to combine our Next Generation Sequencing Service with the WGM technology. The combination of NGS and WGM can be used to order the contigs from a next generation sequencing project against the optical map scaffold. This method is able to highly improve sequencing assemblies. If you are interested in a combined or stand-alone project for WGM, please do not hesitate to contact us.

We look forward to discuss WGM in detail with you.