Tag Archives: technology

Amplicon Sequencing Strategy: What Is Your Technology Of Choice?

amplicon_sequencingWe asked for your favourite technology for amplicon sequencing.

Please find the results here:

  • The majority (41 people) voted for Illumina MiSeq due to the data output
  • 29 prefer Ion Torrent for amplicon sequencing
  • 24 favour Roche 454 because of the long reads
  • 6 people say that classical Sanger sequencing is their technology of choice
  • Just 4 are using other technologies

104 NGS experts took part in the voting.


NGS Moving Towards Forensics

To me the article “Early Adopters Say NGS-based Forensic Testing Could Lead to More Precise Identification” by GenomeWeb (http://bit.ly/WKKIPC) is a very good sign of the “maturation” of the rather young next generation technology. It may find its way into criminal police offices soon.

Currently, forensic profiling is based on STR analysis with capillary electrophoresis, or analysis of mitochondrial DNA with PCR and Sanger sequencing. But next gen sequencing opens up new possibilities. Researchers are now looking at moving both STR profiling and mitochondrial DNA analysis to next gen sequencing, but are also looking to develop SNP-based targeted sequencing panels for forensics, which would enable researchers to identify a person’s ancestry, hair color, or other defining characteristics based on their DNA.

So, what do you think: Will there still be classical DNA profiling or will NGS have taken over in the next decade?

Sequencing Trends beyond Technology

Nice GEN article: They describe forward looking trends they are following.

  1. Sequencing activity polarization.
    It is driven by a series of centralization and decentralization forces that are acting on the space simultaneously.
  2. Workflow value shifts.
    The key drivers of this trend are primarily technical in nature and have to do with the maturation of second-generation technology and the introduction of later-generation sequencers.
  3. Clinical and commercial applications.
    It is characterised by a number of forces working at odds, the two major forces driving this trend – namely increasing demand for higher performance and a rapidly expanding technical performance envelope – are both working together to enhance adoption outside of research.

Read more at GEN >