Polls Archive

What is your preferred medium for secure NGS data shipping?

  • FTP (36%, 9 Votes)
  • Hard drive / CD / USB (36%, 9 Votes)
  • Cloud (28%, 7 Votes)
  • Others (0%, 0 Votes)

Total Voters: 25

Start Date: October 1, 2014 @ 1:40 pm
End Date: November 18, 2014 @ 1:36 pm

What about the new findings about identical twins not having identical genomes?

  • Oh, this was known already. (41%, 14 Votes)
  • It's a problem. Studies relied on them being the same. (24%, 8 Votes)
  • Could be an issue. We have to control for those differences. (18%, 6 Votes)
  • It isn't an issue. They're still very similar. (17%, 6 Votes)

Total Voters: 34

Start Date: August 13, 2015 @ 11:53 am
End Date: March 12, 2015 @ 4:20 pm

Are you ready to have your genome sequenced?

  • Once it's a little bit cheaper (55%, 17 Votes)
  • I already have (23%, 7 Votes)
  • I already know a lot of what it'll tell me (13%, 4 Votes)
  • Maybe just the parts that are actionable (9%, 3 Votes)

Total Voters: 31

Start Date: July 31, 2014 @ 3:15 pm
End Date: No Expiry

In which technology do you see the future of miRNA analysis?

  • Illumina sequencing (38%, 39 Votes)
  • Ion Torrent sequencing (27%, 28 Votes)
  • Nanostring technology (15%, 15 Votes)
  • Microarrays (14%, 14 Votes)
  • qPCR (6%, 6 Votes)

Total Voters: 102

Start Date: March 13, 2014 @ 1:15 pm
End Date: No Expiry

How long have you been involved with next generation sequencing?

  • just now learning about NGS (30%, 55 Votes)
  • 1 - 2 years (21%, 38 Votes)
  • less than 1 year (20%, 37 Votes)
  • 3 - 4 years (18%, 33 Votes)
  • 5 years and more (11%, 20 Votes)

Total Voters: 183

Start Date: October 24, 2013 @ 11:10 am
End Date: March 13, 2014 @ 1:00 pm

Do you use RAD-Seq?

  • No, I’ve never heard of it (50%, 34 Votes)
  • Yes, for genetic marker discovery without reference genome (19%, 13 Votes)
  • Yes, for large genotyping studies (16%, 11 Votes)
  • No, I don’t need it (15%, 10 Votes)
  • Yes, for other reasons (7%, 5 Votes)

Total Voters: 68

Start Date: June 26, 2013 @ 10:17 am
End Date: October 24, 2013 @ 11:10 am

Amplicon sequencing strategy: what is your technology of choice?

  • Illumina MiSeq - due data output (39%, 41 Votes)
  • Ion Torrent (28%, 29 Votes)
  • Roche 454 - due to the long reads (23%, 24 Votes)
  • Classical Sanger sequencing (6%, 6 Votes)
  • Others (4%, 4 Votes)

Total Voters: 104

Start Date: April 22, 2013 @ 9:00 am
End Date: June 26, 2013 @ 10:10 am

What is your favourite high-throughput sequencing machine by platform?

  • Illumina MiSeq (29%, 56 Votes)
  • Illumina HiSeq 2000/2500 (25%, 48 Votes)
  • Roche 454 (19%, 36 Votes)
  • Ion Torrent (16%, 30 Votes)
  • ABI SOLiD (5%, 9 Votes)
  • Pacific Biosciences (4%, 8 Votes)
  • Others (2%, 3 Votes)
  • Illumina Genome Analyzer 2x (0%, 1 Votes)

Total Voters: 191

Start Date: February 22, 2013 @ 2:24 pm
End Date: April 22, 2013 @ 8:25 am

Your opinion about the NGS Expert Blog

  • I like it. Keep on posting! (65%, 30 Votes)
  • Tutorials and basics would be great (17%, 8 Votes)
  • I'd be interested in more news and events (7%, 3 Votes)
  • I'm an expert. Focus on the science! (7%, 3 Votes)
  • I'm interested in best practice (4%, 2 Votes)

Total Voters: 46

Start Date: November 13, 2012 @ 8:24 am
End Date: February 25, 2013 @ 2:14 pm

Do you think it is possible to directly compare the benchtop sequencers MiSeq and IonTorrent with each other?

  • Yes, but results need to be handled with care due to the regular sequencer updates (35%, 12 Votes)
  • No, you would probably miss the strength of one or the other platform (35%, 12 Votes)
  • Maybe, depends on the study protocol (30%, 10 Votes)

Total Voters: 34

Start Date: October 10, 2012 @ 3:39 pm
End Date: November 22, 2012 @ 2:21 pm

Whole Genome Mapping (WGM) with OpGen technology is a new service that improves genome assemblies and is an excellent solution for comparative genomics / strain typing. Have you heard about WGM, yet?

  • Yes, it is a great technology to facilitate genome assembly and for comparative genomics (40%, 22 Votes)
  • Yes, I heard about it, but I am not quite sure about the exact application range (35%, 19 Votes)
  • No I have never heard about it before, but I am interested to learn more (25%, 14 Votes)

Total Voters: 55

Start Date: July 25, 2012 @ 8:55 am
End Date: October 10, 2012 @ 3:00 am

For which kind of application do you use the Roche GS FLX+, the GS FLX Titanium or GS Junior sequencing technology providing read length of up to 700 bp?

  • For a broad range of applications (31%, 11 Votes)
  • Mainly for amplicon sequencing projects (22%, 8 Votes)
  • Mainly for de novo genome sequencing projects (19%, 7 Votes)
  • We have completely switched to Illumina or other sequencing technologies (17%, 6 Votes)
  • Mainly for de novo transcriptome sequencing projects (11%, 4 Votes)

Total Voters: 36

Start Date: May 30, 2012 @ 10:11 am
End Date: July 25, 2012 @ 8:10 am

Nanopore sequencing from Oxford Nanopore sounds really fascinating. What is your opinion regarding this technology?

  • I prefer to wait and check out the real system before judging it. (42%, 22 Votes)
  • I haven't heard about this technology yet (27%, 14 Votes)
  • New NGS technologies always sound great prior launch. I guess that the specifications aren't as excellent as described. (15%, 8 Votes)
  • Nanopore sequencing is really spectacular and I'm sure it will change a lot in the NGS community. (16%, 5 Votes)

Total Voters: 52

Start Date: March 19, 2012 @ 4:07 pm
End Date: May 30, 2012 @ 10:00 am

Which product launches from 2011 will have the most important impact on your research?

  • Launch of the benchtop sequencer Illumina MiSeq (52%, 27 Votes)
  • Increased data output with Illumina sequencing chemistry v3.0 (15%, 8 Votes)
  • Sanger-like read length with Roche GS FLX+ (15%, 8 Votes)
  • Commercial launch of the PacBio RS sequencer (10%, 5 Votes)
  • Launch of new exomes from Illumina, NimbleGen and Agilent (8%, 2 Votes)

Total Voters: 52

Start Date: January 11, 2012 @ 1:36 pm
End Date: March 19, 2012 @ 4:00 am

Which sequencing strategy do you use for scaffolding of contigs?

  • We are sequencing either long paired end (LPE) or long jumping distance (LJD) libraries with a jumping distance of 3, 8, 20 and/or 40 kbp (38%, 11 Votes)
  • We use other strategies, like e.g. BAC end sequencing (21%, 6 Votes)
  • We are sequencing Illumina’s mate pair libraries with a jumping distance of 2-5 kbp (17%, 5 Votes)
  • We use the long PacBio RS reads (14%, 4 Votes)
  • We use a combination of the above mentioned strategies (10%, 2 Votes)

Total Voters: 29

Start Date: November 17, 2011 @ 1:53 pm
End Date: January 11, 2011 @ 1:09 am

Buying instruments or outsourcing the service. What do you prefer?

  • I prefer outsourcing the service to a supplier (50%, 18 Votes)
  • It depends on the project (25%, 9 Votes)
  • Buy instruments to do the sequencing by myself (22%, 8 Votes)
  • I don't know (3%, 1 Votes)

Total Voters: 36

Start Date: September 15, 2011 @ 2:15 pm
End Date: November 16, 2011 @ 2:22 pm

Do you think that PacBio RS sequencing will enter your research within the next year?

  • No, for the near future we will stick to “classical” technologies (41%, 13 Votes)
  • I have no idea yet (22%, 7 Votes)
  • Yes, we are planning to buy the PacBio RS sequencer (19%, 6 Votes)
  • Yes, but we are planning to go via a collaboration or partner group (9%, 3 Votes)
  • Yes, but we are planning to go via a service provider (6%, 2 Votes)
  • We already have performed first experiments (3%, 1 Votes)

Total Voters: 32

Start Date: July 25, 2011 @ 11:40 am
End Date: September 14, 2011 @ 2:09 pm

Which technology will be the methode of choice for high-throughput sequencing in the future?

  • Sequencing through nanopores (31%, 11 Votes)
  • I have no idea yet (22%, 8 Votes)
  • Ion semiconductor sequencing (17%, 6 Votes)
  • Sequencing by synthesis (14%, 5 Votes)
  • DNA nanoball sequencing (11%, 4 Votes)
  • Sequencing by ligation (3%, 1 Votes)
  • Reversible dye terminator sequencing (3%, 1 Votes)
  • Sequencing by hybridization (0%, 0 Votes)
  • Microfluidic Sanger sequencing (0%, 0 Votes)

Total Voters: 36

Start Date: May 4, 2011 @ 7:00 am
End Date: July 25, 2011 @ 11:39 am