Search results: rad-seq

Analysis of oaks using RAD-Seq

Excellent paper just out that dissects the phylogeny of North American oak trees using RAD-Seq. A phylogenetic study of the American Oak clade based on RAD-seq data has recently be published by PLoS ONE. The study is one of the first to demonstrate the utility of RAD-Seq data for inferring phylogeny in a 23-33 million […]

RAD-Seq for Genome Wide Association Studies

Dear NGS Expert Blog reader, To kick off the 2014 discussion on RAD Sequencing for the NGS blog, I wanted to share some results from a recently published study describing the use of RAD for high throughput SNP genotyping in Miscanthus. The tropical grass Miscanthus is an intriguing candidate for bioenergy crop development: It is […]

RAD-Seq Publications in 2013

Dear NGS-Expert Blog reader, As 2013 draws to a close, I wanted to take a few minutes and bring you a some interesting statistics on current trends in RAD sequencing publications and highlight the increasing use of this technology in genomics research. 2013 RAD-Seq Publications in Review Through November 2013, there have been 45 peer-reviewed […]

Do You Use RAD-Seq?

The last months we asked you if you are familiar with RAD-Seq, a new approach combining restriction site associated DNA marker genotyping (RAD) with next generation sequencing technology. 73 people did answer the questions. Nearly the half never heard of it. For this reason we recommend some basic information about RAD-Seq.    

De Novo Genome Sequencing of Sunflower Via RAD-Seq

Dear NGS Expert Blog reader, As part of our ongoing series of posts on RAD sequencing, I wanted to share some results from a recently published study describing the use of RAD-Seq for high throughput SNP development in Helianthus annuus (Sunflower). Sunflower is one of the leading oilseed and confectionery crops in North America, with […]

RAD-Seq Expert Blogger: Welcome Rick Nipper

Dear reader, As a new author here at the NGS Expert Blog, I wanted to take a few minutes to introduce myself, company and the cutting-edge genomics technology my team specializes in. My name is Rick Nipper and I am the President of a company called Floragenex. You can learn a bit more about my […]

RAD-Seq – A brief technical overview

Some time ago I was introducing a new approach combining restriction site associated DNA marker genotyping (RAD) with next generation sequencing technology. Originally this method was developed for microarray platforms. However, the combination of RAD and NGS (Illumina) – resulting in RAD sequencing (RAD-Seq) – enabled the massivly parallel and multiplexed sample sequencing. RAD-Seq is […]

RAD-Seq – A Powerful Tool for Non-Reference Organisms

What is RAD-Seq? Restriction site associated DNA sequencing (RAD-Seq) using the Illumina technology was initially published in PLoS One and in PLoS Genetics. Along with other studies, these studies demonstrated that this smart technique can be very useful for the identification and further analysis of a high number of genetic markers distributed over the genome. This […]

Whose Genome Has Been Sequenced? Cicer Arietinum

With this new bi-weekly series we would like to highlight some if not all genomes that have been sequenced in the last 6 to 12 months. And at this point of time I am still uncertain if the diversity of organisms and species will be the “eye-opener” or the different technologies and strategies that have […]