GS FLX+ Sequencing Successfully Established

Being the first service provider in Germany having our GS FLX sequencers updated to GS FLX+ technology, we have meanwhile successfully established the sequencing of shotgun, long paired end (LPE) and cDNA libraries with GS FLX+. The FLX+ business is fully integrated in the daily routine of our production team and we like to share our experiences on run performance with you:

Of course the read length differs in dependence on the organism to be sequenced, but average read length of 600-700 bp has been proven to be a realistic value for sequencing of shotgun libraries so far. Also the announced yield of at least 1 million reads per full run could be confirmed in practice: All shotgun runs performed so far surpassed this specification.

When sequencing a shotgun library, data output per segment therefore almost doubled, making the Roche 454 sequencing more attractive again. Sanger-like read length are of very high value for any de novo sequencing approach whether for a genome or a transcriptome project.

When you are interested in our FLX+ sequencing service we invite you have a look at our current special GS FLX+ offers.

Regina Dick

About Regina Dick

Regina is a driving supporter of next generation sequencing activities.

One Response to “GS FLX+ Sequencing Successfully Established”

  1. I’ve never really given FLX a good try yet because I feel it seems to have a strange workflow in comparison to other sequencers. But it also seems to be very popular so I’m probably missing out on how you’re supposed to work with it.