Next Generation Sequencing on the Illumina Platform

Are you interested in refreshing your knowledge on the Illumina sequencing technology?

Enjoy the animation that very well illustrates the sequencing of a genomic DNA sample on Illumina platforms. After fragmentation and ligation of adaptors fragments are amplified on a solid surface via bridge PCR. For sequencing reversible terminators are applied in a cyclic way comprising nucleotide incorporation, imaging and cleavage of the blocking group (cyclic reversible termination). Contrary to the way it is presented, the sequencing primer is binding to the upper part of the template and strand elongation is done downwards.

Illumina Sequencing Technology on You Tube